Development An Amperometric Microbial‐enzyme Hybrid Cholesterol Biosensor Based On Ionic Liquid MWCNT Carbon Paste Electrode
Articles > Article · 2021
Authors: Erhan CANBAY, İhsan YAŞA, Erol AKYILMAZ
Electroanalysis, Journal Indexed in SCI Expanded, International, WoS, 2021, vol 33, pp 2381-2391
Summary
Abstract In this study we report the development of an amperometric cholesterol biosensor based on cholesterol oxidase from Pseudomonas sp. and catalase immobilized in carbon paste electrode (CPE) modified with multiwall carbon nanotubes (MWCNT) and ionic liquid (IL). The working electrode (CPE/MWCNT‐IL/Microorganism (MO)‐Catalase) was characterized by impedance spectroscopy and cyclic voltammetry at different stages of its construction. This proposed cholesterol biosensor performed linear relationship in the range of 5–600 μM with a low detection limit of 1.52 μM. The biosensor showed good sensitivity and high selectivity and it was successfully applied for the measurement of cholesterol levels in lyophilized serum samples.