High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
Articles > Article · 2015
Authors: Anup D. Sharma, Pavel A. Brodskiy, Emma M. Petersen, Melih DAĞDEVİREN, Eun-Ah Ye, Surya K. Mallapragada, Donald Sakaguchi
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, Journal Indexed in SCI Expanded, International, 2015
DOI: 10.3791/52242
Summary
Mesenchymal stem cells (MSCs) derived from bone marrow are a powerful cellular resource and have been used in numerous studies as potential candidates to develop strategies for treating a variety of diseases. The purpose of this study was to develop and characterize MSCs as cellular vehicles engineered for delivery of therapeutic factors as part of a neuroprotective strategy for rescuing the damaged or diseased nervous system. In this study we used mouse MSCs that were genetically modified using lentiviral vectors, which encoded brain-derived neurotrophic factor (BDNF) or glial cell-derived neurotrophic factor (GDNF), together with green fluorescent protein (GFP).