Ratlarda oluşturulan pneumoperitoneum modelinde iskemi reperfüzyon hasarının over dokusu üzerine etkileri
Articles > Article · 2021
Authors: İzzet Musayeva, Sabahattin Anıl Arı, Ali AKDEMİR, Fatih OLTULU, Çağdaş ŞAHİN, Dilek TAŞKIRAN, Aylin Buhur, Teksin ÇIRPAN
Ege Tıp Dergisi, National Refreed University Journal, National, TR DİZİN (ULAKBİM), 2021, vol 60, pp 6-12
Summary
<p>Aim: The aims of present study were revealing possible ischemia-reperfusion damage caused by pneumoperitoneum on ovarian tissue and investigating effects of vitamin D on this ovarian damage. Materials and Methods: Twenty-one adult female Sprague Dawley rats were used in this study and these rats were divided into three groups, each contained seven subjects: Sham Group, IschemiaReperfusion and Ischemia-Reperfusion +Vitamin D Group, Ischemia-Reperfusion Group, desufflation was performed 60 minutes after pneumoperitoneum induction and intraabdominal pressure was normalized for 30 minutes. In the Ischemia-Reperfusion + Vitamin D group, pneumoperitoneum induction was performed after 500 mg/kg of vitamin D administration. After the reperfusion period, the ovaries were collected, and ischemia reperfusion damage was evaluated. Histochemical staining was performed to evaluate the follicular degeneration, vascular congestion, inflammation, hemorrhage and edema parameters in the ovaries. In addition, immunohistochemical PCNA (Proliferating cell nuclear antigen) staining was performed to evaluate DNA repair mechanisms. In biochemical analyzes, AMH (Anti-Mullerian hormone), MDA (Malondialdehyde), TAC (Total antioxidant capacity) and SOD (Superoxide dismutase) levels were examined in the ovaries. Results: In histological evaluation, significant differences were found between the groups and also the PCNA values were significantly higher in the Ischemia-Reperfusion Group compared to the Sham and Ischemia-Reperfusion +Vitamin D Groups (p <0.005). Biochemical evaluation revealed that AMH levels measured in Group 2 were significantly lower than Group 1 (p = 0.035), and MDA levels were significantly higher in Group 2 compared to Group 1 (p = 0.006). Conclusion: Results of present study showed that pneumoperitoneum causes severe ischemia reperfusion injury on the ovary. This damage can be reduced with the use of vitamin D. The findings point that vitamin D can be used prophylactically not only in the laparoscopic pneumoperitoneum model, but also before radiotherapy or chemotherapy applications which ovary is directly or indirectly exposed to oxidative stress. <br></p>