Use of pyranose oxidase enzyme in inhibitor biosensing
Articles > Article · 2008
Authors: Idris YAZGAN, Tuba AYDIN, Dilek ODACI, Suna TİMUR
ANALYTICAL LETTERS, Journal Indexed in SCI Expanded, International, 2008, vol 41, pp 2088-2096
DOI: 10.1080/00032710802209276
Summary
An inhibition based biosensing system was developed for the determination of glutathione (GSH) and ethanol (EtOH) as pyranose oxidase (PyOx) inhibitors. The PyOx was immobilized in carbon paste electrode and the amperometric detection of hydrogen peroxide through the enzymatic reaction was monitored at+0.9V versus Ag/AgCl. In addition to the optimization studies, analytical characteristics and the effect of various compounds on the biosensor response were researched. Finally, the proposed system was applied to analyze GSH and EtOH in real matrices.