Antimicrobial and cytotoxic activities of roots of Centaurea cadmea Boiss.
Makaleler > Tam Makale · 2011
Yazarlar: K Alizadeh Astari, Şüra BAYKAN, C Koksal, F Aydın Kose, Canan KARAALP
Planta Medica, Uluslararası, Wos, 2011, cilt 77
Özet
Centaurea cadmea Boiss. is an endemic taxon for Anatolia, growing wild in N, W & SW of Turkey (1). Phytochemical studies revealed the presence of a sesquiterpene lactone, ivalin, which is known cytotoxic compound on several tumor cell lines (2), together with eupatorin, 5-hydroxy-3',4',6,7- tetramethoxyflavone and β-sitosterols from the aerial parts of C. cadmea (3). In vitro anti-inflammatory, antioxidant, antiprotozoal and antimicrobial activities of the aerial parts of C. cadmea extracts have been reported before (4, 5), but no bioactivity study has been performed on roots of the plant, yet. The present study aims at investigating the antimicrobial and cytotoxic activities of roots of C. cadmea . The antimicrobial activities of extracts of the plant were investigated by MIC method. The antibacterial activities of the extracts were tested against four gram negative ( Escherichia coli ATCC 23999, Pseudomonas aeroginosa ATCC 27853, Salmonella typhimurium CCM 5445 and Klebsiella pneumonie CCM 2318) and four gram positive ( Staphylococcus au reus ATCC 6538/P, S. epidermidis ATCC 12228, Enterococcus faecalis ATCC 29212 and Bacillus cereus ATCC 7064) bacteria strains. While the chloroform extract of the plant has no activity against the tested microorganisms, the methanol extract has weak anti-bacterial activity (MIC=64–256µg/ml). The cytotoxic activity were analyzed by cell proliferation assay using WST-1 reagent against three human cancer cell lines; MCF7 (human breast cancer), A549 (human lung cancer), U20S (human osteocarcoma) and one non-cancer cell line; 293HEK (human embriyonic kidney). C. cadmea extract was found active against U2OS (IC 50 : 138µg/ml).