The loss of CD34+cells in peripheral hematopoietic stem cell products cryopreserved by non-controlled rate freezing and stored at-80 degrees C after overnight storage
Makaleler > Tam Makale · 2014
Yazarlar: Ayhan DÖNMEZ, Fergun YILMAZ, Nur SOYER, Seckin CAGIRGAN, Bahar ARIK, Murat TOMBULOĞLU
TRANSFUSION AND APHERESIS SCIENCE, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2014, cilt 51, ss 188-192
DOI: 10.1016/j.transci.2014.08.013
Özet
Although peripheral blood stem cell (PBSC) products cryopreserved by non-controlled rate freezing and stored at -80 degrees C after overnight storage are used frequently, data regarding the rate of loss of CD34+ cells in these products are limited. In this prospective study, CD34+ cells were counted at three (fresh, post-overnight and post-thaw) points in 83 PBSC products from 41 patients by flow cytometry. Compared to fresh products, the mean losses of post-overnight and post-thaw total CD34+ cells are 163% and 38.4% (p = 0.02), and the mean losses of post-overnight and post-thaw viable CD34+ cells are 16.5% and 48.5%, respectively (p < 0.001). The numbers of fresh viable, post-thaw total and post-thaw viable CD34+ cells were inversely correlated with the durations of neutrophil and platelet engraftment. Our results indicate that the mean loss of post-thaw total and viable CD34+ cells is approximately 20% higher than that observed in standard cryopreservation methods. In addition, fresh viable, post-thaw total and especially post-thaw viable CD34+ cell levels are valuable predictors of both neutrophil and platelet engraftments. (C) 2014 Elsevier Ltd. All rights reserved.