A radioanalytical technique for measurement of beta-glucuronidase activities
Articles > Article ยท 2005
Authors: T UNAK, U AVCIBASI, Yeliz YILDIRIM
Journal of Radioanalytical and Nuclear Chemistry, Journal Indexed in SCI Expanded, International, WoS, 2005, vol 266, pp 503-506
DOI: 10.1007/s10967-005-0939-7
Summary
Phenolphthalein-glucuronide is a commonly used glucuronide conjugate for beta-glucuronidase measurements. The quantity of phenolphthalein liberated by beta-glucuronidase is measured spectrophotometrically. The detection limit of the quantity of phenolphthalein using spectrophotometry is a few mu g. In this study, a new radioanalytical technique for the measurement of beta-glucuronidase was applied which is 10(6) times more sensitive than the spectrophotometric technique. Radioiodinated phenolphthalein-glucuronide and phenyl-N-glucuronide were used in this study in which the beta-glucuronidase levels of some tissue samples were measured.