Impedimetric biosensor for cancer cell detection
Makaleler > Tam Makale · 2013
Yazarlar: Basak SEVEN, Mariem BOUROUROU, Kamal ELOUARZAKİ, Jean Francois CONSTANT, Chantal GONDRAN, Michael HOLZİNGER, Serge COSNİER, Suna TİMUR
ELECTROCHEMISTRY COMMUNICATIONS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2013, cilt 37, ss 36-39
DOI: 10.1016/j.elecom.2013.10.003
Özet
Electrochemical impedance spectroscopy was evaluated for the label free detection of MCF-7 cancer cell in which c-erbB-2 receptor is overexpressed on the cell surfaces. Anti-c-erbB-2, used as a specific antibody, was immobilized on electrogenerated polypyrrole-NHS on electrodes via covalent linking. The polymer formation, the grafting of the antibody, and the recognition event with the cancer cells using MCF-7 as a model cell line, were characterized by using cyclic voltammetry and fluorescence microscopy. The impedimetric sensor showed high sensitivity from 100 to 10 000 cell/mL without needing any labeling step and represents an efficient transduction method for cell selective detection. (C) 2013 Elsevier B.V. All rights reserved.