The Enhancement of Polygalacturonase and Polymethylgalacturonase Production on Solid-State Conditions byAspergillus foetidus
Articles > Article · 2008
Authors: Evrim Taskin, Rengin ELTEM
Food Biotechnology, Journal Indexed in SCI Expanded, International, WoS, 2008, vol 22, pp 203-217
DOI: 10.1080/08905430802262533
Summary
In this study, a previously isolated strain of Aspergillus foetidu s Ege-K-635 producing highly active polygalacturonase and polymethylgalacturonase was used for improvement of enzyme production using solid-state conditions. To enhance the enzyme yield, the mixture of two substrates such as sugar beet pulp and wheat bran, having different ratios of carbon-nitrogen and moisture levels was used. Further the effect of the amount of inoculum was investigated. Physical parameters for the highest polygalacturonase and polymethylgalacturonase production under solid state conditions were determined on wheat bran: the sugar beet pulp (1:2) mixture substrate composition having a 31:1 carbon-nitrogen ratio with 8 × 106 spores/gram of dry substrate as the inoculum amount. Adequate moisture levels were 75% and 70% for polygalacturonase and polymethylgalacturonase, respectively. Under these conditions, the highest activities obtained were 385 ± 12.3 Ug−1 on the third day for polygalacturonase and 18.3 ± 3.2 Ug−1 on the second day for polymethylgalacturonase.