Hematopoietic Stem Cell Transplantation Using Preimplantation Genetic Diagnosis and Human Leukocyte Antigen Typing for Human Leukocyte Antigen-Matched Sibling Donor: A Turkish Multicenter Study
Makaleler > Tam Makale · 2017
Yazarlar: Emin KÜREKÇİ, Alphan Kupesiz, Sema Anak, Gulyuz ÖZTÜRK, Orhan GÜRSEL, Serap AKSOYLAR, Talia Ileri, Baris Kuskonmaz, Ibrahim EKER, MUALLA ÇETİN, Gulsun Tezcan Karasu, Zuhre Kaya, Tunc Fisgin, Mehmet Ertem, Savaş KANSOY, Mehmet Akif Yesilipek
BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2017, cilt 23, ss 790-794
DOI: 10.1016/j.bbmt.2017.02.002
Özet
Preimplantation genetic diagnosis involves the diagnosis of a genetic disorder in embryos obtained through in vitro fertilization, selection of healthy embryos, and transfer of the embryos to the mother's uterus. Preimplantation genetic diagnosis has been used not only to avoid the risk of having an affected child, but it also offers, using HLA matching, preselection of potential HLA-genoidentical healthy donor progeny for an affected sibling who requires bone marrow transplantation. Here, we share the hematopoietic stem cell transplantation results of 52 patients with different benign and malign hematological or metabolic diseases or immunodeficiencies whose donors were siblings born with this technique in Turkey since 2008. The median age of the patients' at the time of the transplantation was 8 years (range, 3 to 16 years) and the median age of the donors was 2 years (range,.5 to 6 years). The most common indication for HSCT was thalassemia major (42 of all patients, 80%). The stem cell source in all of the transplantations was bone marrow. In 37 of the transplantations, umbiliCal cord blood of the same donor was also used. In 50 of the 52 patients, full engraftment was achieved with a mean of 4.6 x 10(6) CD 34(+) cells per kg of recipient weight. Ninety-six percent of the patients have been cured through hematopoietic stem cell transplantation without any complication. Primary engraftment failure was seen in only 2 patients with thalassemia major. All of the donors and the patients are alive with good health status. Preimplantation genetic diagnosis with HLA matching offers a life-saving chance for patients who need transplantation but lack an HLA genoidentical donor. (C) 2017 American Society for Blood and Marrow Transplantation.