Photoelectrocatalytic determination of NADH in a flow injection system with electropolymerized methylene blue
Makaleler > Tam Makale · 2010
Yazarlar: YUSUF DİLGİN, Didem Giray Dilgin, Zekerya DURSUN, Hatice İsmet GÖKÇEL, Delia GLIGOR, Burcu Bayrak, Bensu Ertek
Electrochimica Acta, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2010, cilt 56, ss 1138-1143
DOI: 10.1016/j.electacta.2010.10.084
Özet
It was firstly described that a glassy carbon electrode electropolymerized with methylene blue shows an efficient photoelectrocatalytic activity towards NADH oxidation in a phosphate buffer solution (pH 7.0). In order to perform the photoelectrocatalytic determination of NADH in a flow injection analysis (FIA) system, a home-made flow electrochemical cell with a suitable transparent window for the irradiation of the electrode surface was constructed. The currents obtained from the photoamperometric measurements in the FIA system at optimum conditions (flow rate of carrier solution, 1.3 mL min(-1): transmission tubing length, 10 cm; injection volume, 100 mu L; and constant applied potential, +150 mV vs. Ag/AgCl) were linearly dependent on the NADH concentration and linear calibration curves were obtained in the range of 1.0 x 10(-7)-2.0 x 10(-4) M. The detection limit was found to be 4.0 x 10(-8) M for photoamperometric determination of NADH. (C) 2010 Elsevier Ltd. All rights reserved.