Modification of Polydivinylbenzene Microspheres by a Hydrobromination/Click‐Chemistry Protocol and their Protein‐Adsorption Properties
Makaleler > Tam Makale · 2010
Yazarlar: Yasemin Yuksel Durmaz, Bünyamin Karagöz, Niyazi Bicak, Dilek ODACI, Esra Evrim YALÇINKAYA, Suna TİMUR, Yusuf Yağcı
Macromolecular Bioscience, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2010, cilt 11, ss 141-150
Özet
Hydrophobic- and/or hydrophilic-polymer-grafted PDVB microspheres are synthesized by the combination of hydrobromination and click-chemistry processes. The modified-PDVB microspheres and the intermediates at various stages of synthesis are characterized using GPC, ¹H NMR and FTIR spectroscopy and TGA analysis. Use of the microspheres as a support matrix for reversible protein immobilization via adsorption is investigated. The system parameters such as the adsorption conditions (i.e., enzyme concentration, medium pH) and desorption are studied and evaluated with regards to the biocatalytic activity and adsorption capacity.