Quantitative and cytotoxic activity determinations on Galanthus nivalis subsp. cilicicus
Articles > Article · 2005
Authors: Gülen İrem KAYA, BELKIS GÖZLER
Fitoterapia, Journal Indexed in SCI Expanded, International, WoS, 2005, vol 76, pp 340-343
DOI: 10.1016/j.fitote.2005.02.003
Summary
Aerial and underground parts of Galanthus nivalis subsp. cilicicus, a wild-growing species in Turkey, were collected during two different vegetation periods in flowering and fruiting seasons. Herba and bulbus Galanthi were prepared from each specimen. With the aim of collecting data for prospective monographs on this drug, contents of humidity, ash, sulphated ash and total alkaloids were determined according to DAB 10. The specimens were also analyzed quantitatively for two of the principal alkaloids of the genus, galanthamine and lycorine, by using a method based on spectrophotometry complemented with TLC. LC50 values were determined for the ethanolic and alkaloidal extracts of each of the specimens using brine shrimp lethality bioassay. (c) 2005 Elsevier B.V All rights reserved.