From Scarcity to Survival: Adaptive Strategies of A549 Lung Cancer Cells Under Serum Starvation
Makaleler > Tam Makale · 2025
Yazarlar: Gunel Mukhtarova, Cagla Ozdenizer, Deniz Ece, Ufuk MERT, Ayşe CANER
Thoracic Research and Practice, Uluslararası, Scopus, 2025, cilt 26, ss 21-22
DOI: 10.4274/thoracrespract.2025.s008
Özet
INTRODUCTION: . MATERIAL AND METHODS: A549 cells were cultured under different serum concentrations [1%, 10%, and 20% fetal bovine serum (FBS)], with cells maintained in 10% FBS serving as the control group. To evaluate the effects of serum availability on cellular behavior, various assays were performed, including Giemsa staining for morphology, wound healing assay for migration, flow cytometric analysis of CD44 and CD133 for stemness, XTT assay with flavopiridol for drug resistance, colony formation assay for clonogenic capacity, three-dimensional spheroid formation assay, and reverse transcription quantitative polymerase chain reaction (RT-qPCR) for gene expression profiling. RESULTS: tumor biology models. CONCLUSION: model for studying metabolic flexibility and therapy resistance mechanisms in lung cancer cells.