Trombinin Alginat Jeline İmmobilizasyonu ve İmmobilize Trombinin In Vitro Uygulaması
Makaleler > Tam Makale
Yazarlar: Akkaya, Alper
DergiPark (Istanbul University), Uluslararası
Özet
Bleeding is one of the biggest problems of humanity. Blood loss in road traffic accidents and surgical operations is an important cause of death. In this study, a product which could decrease bleeding time was developed. The production process of developed product was very simple and the product has commercialization potential. Thrombin was immobilized to calcium alginate beads via ionic interactions. In this method, negative charged carboxylate groups of calcium alginate interacted ionically with the positive charge of thrombin. Optimization studies of thrombin immobilization were performed for increasing immobilization efficiency and it was found to be 4.1% at the end of optimization studies. The thrombin immobilized calcium alginate beads were used for the recalcification time test. The recalcification time is an indicator of bleeding time. The thrombin immobilized calcium alginate lowered recalcification time to 50 percent. Developed product has a potential to stop bleeding.