IMMOBILIZATION of THROMBIN to ALGINATE GEL and in vitro APPLICATION of IMMOBILIZED THROMBIN. (TROMBİNİN ALGİNAT JELİNE İMMOBİLİZASYONU ve İMMOBİLİZE TROMBİNİN in vitro UYGULAMASI)
Makaleler > Tam Makale · 2018
Yazarlar: Alper AKKAYA
Hacettepe Journal of Biology and Chemistry, Uluslararası, 2018, cilt 45, ss 287-294
Özet
Bleeding is one of the biggest problem of humanity. Blood loss in road traffic accidents and surgical operations is an important cause of death. In this study, a product which could decrease bleeding time was developed. The production process of developed product was very simple and the product has commercialization potential. Thrombin was immobilized to calcium alginate beads via ionic interactions. In this method, negative charged carboxylate groups of calcium alginate interacted ionically with the positive charge of thrombin. Optimization studies of thrombin immobilization were performed for increasing immobilization efficiency and it was found to be 4.1% at the end of optimization studies. The thrombin immobilized calcium alginate beads were used for the recalcification time test. The recalcification time is an indicator of bleeding time. The thrombin immobilized calcium alginate lowered recalcification time to 50 percent. Developed product has a potential to stop bleeding. Kanama, insanligin en buyuk problemlerinden biridir. Trafik kazalari ve cerrahi operasyonlardaki kan kaybi, olumun onemli bir sebebidir. Bu calismada, kanama suresini azaltabilecek bir urun gelistirildi. Gelistirilen urunun uretim sureci cok basitti ve urunun ticarilesme potansiyeli bulunmaktadir. Trombin, iyonik etkilesimler yoluyla kalsiyum aljinat boncuklara immobilize edildi. Bu yontemde, kalsiyum aljinatin negatif yuklu karboksilat gruplari, trombinin pozitif yukleriyle iyonik olarak etkilesime girdi. Immobilizasyon verimini arttirmak icin trombin immobilizasyonunun optimizasyon calismalari yapildi ve optimizasyon calismalarinin sonunda immobilizasyon verimi %4,1 olarak bulundu. Trombin immobilize kalsiyum aljinat boncuklari, rekalsifikasyon zamani testi icin kullanildi. Rekalsifikasyon zamani, kanama suresi icin bir indikatordur. Trombin immobilize kalsiyum aljinat, rekalsifikasyon zamanini %50'ye dusurdu. Gelistirilen urun kanamayi durdurma potansiyeline sahiptir.