Antimicrobial activity, cg/ms analysis and cytotoxıc potential of essential oil of bergamot (citrus bergamia risso et poiteau) peel
Makaleler > Tam Makale · 2024
Yazarlar: Dilek Keskin, Nur CEYHAN GÜVENSEN, Mehlika Alper, Hatice Güneş
INTERNATIONAL JOURNAL OF APPLIED BIOLOGY AND ENVIRONMENTAL SCIENCE (IJABES), Uluslararası, 2024, cilt 6, ss 1-13
DOI: 10.5505/ijabes.2024.35220
Özet
In this study, bergamut (Citrus bergamia Risso et Poiteau) essential oil (BEO) extracted from bergamut peel was examined for antimicrobial effects, volatile compounds, and cytotoxic properties against certain cancer cell lines.Disc diffusion and microdilution methods were orderly used to determine antimicrobial and the minimum inhibitory concentration (MIC) values of BEO.Cytotoxic activity was revealed using MTT assay.Among the six bacteria tested, only Bacillus subtilis and Staphylococcus aureus showed the highest antimicrobial activity, in comparision with erytromycin and ampicilline positive controls.BEO exhibited antimicrobial activity against Candida albicans as well.In addition, MIC value was only observed for Escherichia coli (8 mg/ml) and C. albicans (1.6 mg/ml).The GC/MS analyses showed the presence of 18 compounds in BEO.The main constituents were found to be linalool (46.58%), limonene (22.32%) and linalyl acetate (15.46%).BEO at 0.06% resulted in different cytotoxicities against MCF-7 (85%), A549 (78%) and HeLa (70%) cells.Jurkat cells were more resistant against BEO because only 38% cytotoxicity was observed at the same concentration of BEO.In conclusion, BEO from Turkey has potential source of active compounds for development of therapeutic and preventive strategies as well as cancer disease.