Immobilization of Phospholipase A2on Porous Glass and Its Application for Lowering Serum Cholesterol Concentration
Articles > Article · 2003
Authors: Mustafa Teke, Seçil ÖNAL, Ali KILINÇ, AZMİ TELEFONCU
Artificial Cells, Blood Substitutes, and Biotechnology, Journal Indexed in SCI Expanded, International, WoS, 2003, vol 31, pp 467-478
Summary
Phospholipase A2 (PLA2; EC 3.1.1.4) is a lipolytic enzyme that hydrolysis the ester bond in sn-2 position of phospholipids. In this work, the PLA2 from hog pancreas was covalently coupled to porous glass. The properties of free and immobilized enzyme were also investigated and compared. The optimum pH and temperature were found as 8.5 and 50 degrees C, respectively for both free and immobilized enzyme. The immobilized enzyme had good properties that potential for medical application is considerable. Its use in lowering plasma cholesterol concentrations in blood samples was also demonstrated.