Rapid, Accurate and Low-Cost Innovative Approach for Molecular Identification of Aspergillus species
Articles > Article · 2021
Authors: Yüksel GEZGİN, Sibel Arslan, Zeycan Nurçe
The Journal of Fungus /Mantar Dergisi, Refereed Journals of Other Institutions, International, TR DİZİN (ULAKBİM), 2021, vol 12, pp 113-121
Summary
<p>Rapid, Accurate and Low-Cost Innovative Approach for Molecular Identification of Aspergillus species Abstract: Some of Aspergillus species are the most common food spoilage fungi contaminating economically important agricultural products. They also produce carcinogenic, teratogenic and mutagenic mycotoxins that threaten human health. For all these reasons, precise and accurate identification of Aspergillus species is of great importance. In this study, the Mycelium Tissue- Polymerase Chain Reaction (MT-PCR) based molecular technique was developed and therefore the genomic DNA isolation step which used as a template in the Polymerase Chain Reaction was eliminated. The mycelium tissue PCR-based molecular technique has some advantages. For example, it does not require expensive and specialized equipment, not require expensive chemicals such as proteinase K, RNAse, other enzymes and not use toxic chemicals such as fenol/kloroform during the process of DNA isolation. The classic genomic DNA extraction procedure based on mycelium from liquid cultivations is also relatively time-consuming. For molecular identification of Aspergillus isolates were amplified two different gene regions (ß-tubulin: benA, Calmodulin: CaM) by using tissue samples in this study. Then, all PCR products were purified and were sent to base sequence analysis. Sequence analysis results were examined using bioinformatic tools and the isolates were identified to species level as Aspergillus tubingensis R. Mosseray. </p><p>Key words: Aspergillus, Calmodulin Gene, ?eta-Tubulin Gene, Polymerase Chain Reaction<br></p>