Polyphenol-rich pimenta dioica extract induces mitochondrial apoptosis in ovarian cancer cells
Makaleler > Tam Makale · 2026
Yazarlar: Duygu Döndü AYGÜNEŞ JAFARI
Ege Tıp Dergisi, Uluslararası, 2026, cilt 65, ss 91-98
DOI: 10.19161/etd.1817431
Özet
Aim: Ovarian cancer is a highly lethal gynecologic malignancy, accounting for more deaths than any other female reproductive cancer. Standard chemotherapy often fails over time due to chemoresistance and toxic side effects, resulting in poor five-year survival rates under 40%. There exists an increasing scholarly interest in phytochemicals as potential anticancer agents, attributable to their multifaceted mechanisms of action and comparatively reduced toxicity profiles. Pimenta dioica (allspice) berries are rich in antioxidants and contain bioactive compounds such as eugenol and gallic acid with reported antiproliferative properties. However, their effects in ovarian cancer remain underexplored. Materials and Methods: P. dioica extract was evaluated on human ovarian adenocarcinoma OVCAR-3 cells. Cytotoxicity was assessed by WST-1 viability assays across a 12.5-200 µg/mL dose range (48-72 h exposure) to determine IC₅₀ values. Apoptosis induction was analyzed by Annexin V-FITC /PI via flow cytometry. Expression of apoptosis related genes (BAX, BCL-2, CASP3, CASP9 and TP53) was quantified by qRT-PCR. Results: P. dioica extract inhibited OVCAR-3 cell viability in dose and time dependent manner (p