Effects of Hypericum perforatum extract on 6-hydroxydopamine neurotoxicity in differentiated SH-SY5Y cells
Makaleler > Tam Makale · 2023
Yazarlar: Baris Bitmez, Seda Gultekin, Irem Albayrak, Yigit Deveci, Yusuf Sicak, Emine Akalin, Ademi Fahri PİRHAN, Ulas Gurer, Belkis Arslan
EGYPTIAN PHARMACEUTICAL JOURNAL, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2023, cilt 22, ss 188-191
Özet
Background and objective Parkinson's disease (PD) is the second most common neurodegenerative disease after Alzheimer's disease. In our study, PD model was created as a result of exposure to 6-hydroxydopamine (6-OHDA) in SH-SY5Y cells, which is a human neuroblastoma cell line. The protective effect of Hypericum perforatum on PD was investigated. Materials and methods Phytochemical analysis of H. perforatum extract was performed. Then, SH-SY5Y cells were differentiated using retinoic acid and then administered 6-OHDA neurotoxin. To determine the protective effects of H. perforatum extract, we investigated the changes in the mRNA expression level of caspase-3, total oxidant status, and antioxidant levels in differentiated SH-SY5Y. Results and conclusion According to our results, H. perforatum extract contains glycosides, tannins, flavonoids, and carbohydrates as the major secondary metabolites. H. perforatum extract significantly reduced caspase-3 gene expression against 6-OHDA toxicity in differentiated SH-SY5Y cells. It was found that total oxidant status level increased significantly in the 6-OHDA experimental group compared with the control and H. perforatum experimental groups. It was found that H. perforatum extract has an inhibitory effect on caspase-3 gene expression, which plays an important role in apoptosis. Therfore, H. perforatum extract has been shown to have a therapeutic potential against 6-OHDA toxicity.