Analysis of vitamin A in eggs by high pressure liquid chromatography
Articles > Article · 1991
Authors: Semih ÖTLEŞ, Y HISIL
Food / Nahrung, Journal Indexed in SCI Expanded, International, 1991, vol 35, pp 391-394
Summary
Abstract A high speed liquid chromatographic method was described for the rapid determination of vitamin A in eggs. After saponification and extraction, the sample was separated on a reversed‐phase column (C18) with acetonitrile ‐ methylene‐chloride ‐ methanol as the mobile phase. Quantitative determination was accomplished by monitoring the ultraviolet absorbance of retinol at 280 nm. Recovery of vitamin A was 97.1 %.