Influence of the Reactant Concentrations on the Binding Mode of α‐Amylase
Makaleler > Tam Makale · 1984
Yazarlar: E. Çörüşlü, B. Pekin
Starch - Stärke, Uluslararası, 1984, cilt 36, ss 91-93
Özet
Abstract Carboxymethylcellulose activated to varying extents by cyanogen bromide was used in insolubilizing Bacillus subtilis α‐amylase. The retention of native activities in the resulting derivatives toward soluble starch and the amounts of bound enzyme were determined. The effect of enzyme concentration in the coupling medium on the retention of activity was also investigated. The results are shown to accord with the hypothesis that the enzyme molecules tend to concentrate more on the surface of the carrier than inside the pores as the reactivities of the components in the coupling reaction are increased.