Enrichment and Isolation of Anoxygenic Phototrophic Bacteria in Winogradsky Column

Articles > Article · 2006

Authors: İhsan YAŞA, Bilge Hilal Cadirci, Ali KOÇYİĞİT, Tansel Ozturk

SU URUNLERI DERGISI, Journal Indexed in ESCI, International, WoS, 2006, vol 23, pp 71-73

Summary

The aim of the study is to isolate the anoxygenic photothophic bacteria from mud and soil obtained from Denizli Saraykoy and to determine bacteriochlorophyll content of the isolate. For this purpose samples were enriched under sunlight in Winogradsky column at room temperature for 2-3 months. Pure culture was obtained by decimal dilutions in Pfennig's medium and screened for maximum adsorption spectrum between 400-1100 nm. The absorption maxima of cell suspensions of isolated strains are 506-518, 806-810 and 854-865 nm.

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