Electrochemical aptasensing reveals serum myelin alterations in diabetic neuropathy

Makaleler > Tam Makale · 2026

Yazarlar: Marina Serin, Arman VAHABİ, Derviş BİRİM, Güliz ARMAGAN, Nezih SERTÖZ, Pınar KARA, Anıl Murat ÖZTÜRK

ANALYTICAL BIOCHEMISTRY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2026, cilt 716, ss 116156-116156

DOI: 10.1016/j.ab.2026.116156

Özet

<p>Diabetic&nbsp;neuropathy&nbsp;(DN)&nbsp;is&nbsp;a&nbsp;complex&nbsp;neuropathic&nbsp;process&nbsp;in&nbsp;the&nbsp;peripheral&nbsp;nervous&nbsp;system&nbsp;caused&nbsp;by&nbsp;diabetesinduced&nbsp;hyperglycemia&nbsp;and&nbsp;its&nbsp;related&nbsp;pathways.&nbsp;Early-stage&nbsp;diagnostic&nbsp;methods&nbsp;to&nbsp;detect&nbsp;nerve&nbsp;damage&nbsp;in&nbsp;diabetic&nbsp;patients&nbsp;remain&nbsp;limited,&nbsp;hindering&nbsp;timely&nbsp;initiation&nbsp;of&nbsp;treatment.&nbsp;Delays&nbsp;in&nbsp;early&nbsp;diagnosis&nbsp;of&nbsp;neuropathy&nbsp;can&nbsp;exacerbate&nbsp;the&nbsp;disease,&nbsp;leading&nbsp;to&nbsp;diabetic&nbsp;foot&nbsp;ulcers&nbsp;and&nbsp;ultimately,&nbsp;amputations.&nbsp;Nerve&nbsp;damage&nbsp;in&nbsp;cases&nbsp;involving&nbsp;demyelination&nbsp;is&nbsp;associated&nbsp;with&nbsp;increase&nbsp;in&nbsp;serum&nbsp;myelin&nbsp;basic&nbsp;protein&nbsp;(MBP)&nbsp;levels.&nbsp;In&nbsp;this&nbsp;study,&nbsp;we&nbsp;developed&nbsp;electrochemical&nbsp;aptasensor-based&nbsp;prototype&nbsp;diagnostic&nbsp;method&nbsp;for&nbsp;the&nbsp;rapid,&nbsp;sensitive,&nbsp;specific,&nbsp;and&nbsp;low-cost&nbsp;Point-of-Care&nbsp;analysis&nbsp;of&nbsp;MBP.&nbsp;Disposable&nbsp;graphite&nbsp;electrodes&nbsp;modified&nbsp;with&nbsp;functionalized&nbsp;graphene&nbsp;oxide&nbsp;nanomaterial&nbsp;were&nbsp;utilized&nbsp;as&nbsp;the&nbsp;sensor&nbsp;surface,&nbsp;with&nbsp;immobilized&nbsp;MBP-specific&nbsp;aptamer&nbsp;forming&nbsp;the&nbsp;bioactive&nbsp;binding&nbsp;layer.&nbsp;At&nbsp;first,&nbsp;MBP&nbsp;binding&nbsp;in&nbsp;serum&nbsp;samples&nbsp;was&nbsp;monitored&nbsp;using&nbsp;electrochemical&nbsp;impedance&nbsp;spectroscopy.&nbsp;Following&nbsp;the&nbsp;validation&nbsp;of&nbsp;aptasensors,&nbsp;serum&nbsp;samples&nbsp;from&nbsp;14&nbsp;healthy&nbsp;volunteers&nbsp;and&nbsp;16&nbsp;patients&nbsp;with&nbsp;type&nbsp;II&nbsp;diabetes&nbsp;mellitus&nbsp;(with&nbsp;and&nbsp;without&nbsp;DN)&nbsp;were&nbsp;analyzed.&nbsp;A&nbsp;statistically&nbsp;significant&nbsp;difference&nbsp;in&nbsp;serum&nbsp;MBP&nbsp;levels&nbsp;was&nbsp;observed&nbsp;between&nbsp;healthy&nbsp;volunteers&nbsp;and&nbsp;diabetic&nbsp;patients,&nbsp;demonstrating&nbsp;the&nbsp;diagnostic&nbsp;potential&nbsp;of&nbsp;the&nbsp;method.&nbsp;The&nbsp;aptasensor-based&nbsp;method&nbsp;achieved&nbsp;a&nbsp;limit&nbsp;of&nbsp;detection&nbsp;(LOD)&nbsp;of&nbsp;0.35&nbsp;ng/mL,&nbsp;with&nbsp;a&nbsp;clinical&nbsp;application&nbsp;range&nbsp;from&nbsp;0.35&nbsp;to&nbsp;128&nbsp;ng/mL.&nbsp;As&nbsp;a&nbsp;proof-of-concept,&nbsp;the&nbsp;proposed&nbsp;aptasensor-based&nbsp;method&nbsp;showed&nbsp;a&nbsp;good&nbsp;agreement&nbsp;with&nbsp;ELISA&nbsp;in&nbsp;detecting&nbsp;MBP&nbsp;levels&nbsp;in&nbsp;serum&nbsp;samples.&nbsp;This&nbsp;study&nbsp;highlights&nbsp;the&nbsp;potential&nbsp;of&nbsp;the&nbsp;aptasensor-based&nbsp;method&nbsp;as&nbsp;a&nbsp;promising&nbsp;tool&nbsp;for&nbsp;early&nbsp;and&nbsp;accurate&nbsp;diagnosis&nbsp;of&nbsp;diabetic&nbsp;neuropathy&nbsp;and&nbsp;other&nbsp;conditions&nbsp;when&nbsp;MBP&nbsp;levels&nbsp;are&nbsp;altered.</p>

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