Lectin attached affinity cryogels for amyloglucosidase adsorption
Articles > Article · 2018
Authors: Melis Bayraktaroğlu, Hande Orhan, Sinem Evli, Sinan Akgöl, Deniz Aktaş Uygun, Murat Uygun
Journal of Carbohydrate Chemistry, International, WoS, 2018, vol 37, pp 302-317
DOI: 10.1080/07328303.2018.1487972
Summary
Preparation of poly[ethylene glycol dimethacrylate (EGDMA)-glycidyl methacrylate (GMA)] cryogel and its usability for amyloglucosidase adsorption were investigated. Cryogels were prepared by cryocopolymerization technique and then functionalized by lectin concanavalin A (Con A). The synthesized cryogel was characterized by FTIR, SEM and EDX analysis and then used for adsorption of amyloglucosidase in a continuous flow system. The maximal amyloglucosidase adsorption efficiency of Con A attached cryogel was found to be as 30.50 mg/g cryogel. Con A modified cryogels were used repeatedly for 30 times without any significant decrease at the amyloglucosidase adsorption capacity. SDS-PAGE and activity studies confirmed that the desorbed amyloglucosidase was active and retained 90% of its initial activity after the adsorption/desorption processes.