Optical determination of glucose with glucose oxidase immobilized in PVC together with fluorescent oxazol-5-one derivatives
Articles > Article · 2008
Authors: Gulsiye Ozturk, Suna TİMUR, Serap Alp
ANALYTICAL LETTERS, Journal Indexed in SCI Expanded, International, 2008, vol 41, pp 608-618
DOI: 10.1080/00032710801910551
Summary
Fully reversible biosensors for glucose monitoring based on solid polyvinylchloride (PVC) films where the enzyme glucose oxidase (GOx) was incorporated together with 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadec yl)benzylidene]-5-oxazolone (CPO-1), 2-(3,5-dinitrophenyl)-4-[4-(1,4,7,10- tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO-2), 2-( 4- nitrophenyl) -4-[ 4-( 1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO-3) and 2-(4-tolyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO-4) derivatives have been developed. The limit of detection (LOD) values for glucose were found to be 1.47 x 10 25 M, 2.01 x 10 25 M, 0.89 x 10 25 M, and 0.12 x 10 25 M for CPO-1, CPO-2, CPO-3, and CPO-4, respectively (n = 7). Sensor films were found to have excellent photostability.