Comparative Gene Expression Profiles of Leishmania major and Leishmania infantum Promastigotes
Makaleler > Tam Makale · 2021
Yazarlar: Özlem Ulusan Bağcı, aygül sadıqova, Ayşe CANER
Turkish Journal of Parasitology, Diğer Kurumların Hakemli Dergileri, Uluslararası, Scopus, 2021, cilt 45, ss 88-94
DOI: 10.4274/tpd.galenos.2021.66375
Özet
Objective: promastigotes through comparative analysis of gene expressions. Methods: (MHOM/MA/67/ITMAP/263) cell lines was performed. Afterwards, total RNA isolation and cDNA synthesis were performed and fold changes in the expression levels of 30 genes that play a role in metabolic pathways and nucleic acid synthesis and co-expressed in two species were evaluated by reverse transcriptase polymerase chain reaction. Functions of genes were determined using LeishDB and KEGG databases. Results: (fold change >1). These genes include phosphoglycan beta 1.3 galactosyltransferase-like, lathosterol oxidase-like, fatty acid elongase, 3-oxo-5 alpha-steroid 4-dehydrogenase, calpain-like cysteine peptidase, acetyl-coA synthetase, 3'-nucleotidase/nuclease, 3'-nucleotidase/nuclease precursor and 3-ketoacyl-coA thiolase-like. When the functions of the proteins that correspond to the genes common in the two species were examined in detail using the databases, it was determined that these genes play role in lipid, protein, carbohydrate and nucleic acid metabolic functions of the parasite. Conclusion: species may cause differences in the virulence, pathogenesis, clinical features and treatment modality between these parasite species. In addition, evaluation of gene profiles is important in the selection of species-specific or common targets for vaccine and drug studies.