Investigation of production efficiency of poly-β-hydroxybutyrate (PHB) by marine, bioluminescent and indigenous vibrio spp
Makaleler > Tam Makale · 2014
Yazarlar: Esra ERSOY ÖMEROĞLU, Burcu Barutoglu, Ismail Karaboz
Fresenius Environmental Bulletin, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2014, cilt 23, ss 1959-1970
Özet
Bioluminescent bacteria isolated from marine samples were investigated for their ability of poly-β-hydroxy-butyrate (PHB) accumulation. Five isolates SeLu25, S3W15, E-19, H-15 and H-19 were obtained from sediment, seawater, internal area of Serranus hepatus, Citharus linguatula and Conger conger, respectively. SeLu25, S3W15 and E-19 were identified as Vibrio gigantis based on partial 16S rRNA sequence homology and the other (H-15 and H-19) as V. harveyi and V. crassostreae, respectively. The maximum (V. harveyi H-15 strain) and the minimum (V. gigantis E-19 strain) PHB producers were determined. To increase the production efficiency on PHB production by bioluminescent Vibrio species, the effects of carbon sources, organic and inorganic nitrogen sources, pH, sodium chloride concentration, incubation time and agitation rate were evaluated. The highest PHB content (72.57%) was materialized by V. harveyi H-15 strain in marine basal medium (MBM) with 3% of glycerol, pH 7, 1.9% of NaCl by shaking at 100 rpm after 36 h incubation when it was in stationary phase. Elongation of incubation time and increasing of agitation rate influenced negatively PHB production. V. gigantis E-19 strain produced PHB in ratio of 8.72% under these optimized conditions. V. harveyi H-15 strain has the highest productivity of PHB among all bioluminescent genus and strains. So, the study highlights that V. harveyi H-15 strain; fast growth, high PHB producer (72.57% of cell dry weight) and bioluminescent; may be utilizable property for the industrial production of biodegradable and biocompatible material, PHB. © by PSP.