A novel non-competitive amperometric immunosensor by using thiourea-glutaraldehyde-modified gold electrode for immunoglobulin M detection
Makaleler > Tam Makale · 2013
Yazarlar: Erol AKYILMAZ, Erhan DİNÇKAYA
Artificial Cells, Nanomedicine, and Biotechnology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2013, cilt 41, ss 389-394
DOI: 10.3109/21691401.2012.761227
Özet
A novel non-competitive amperometric immunosensor based on a self-assembled monolayer (SAM) of thiourea modified by a polymeric Schiff's base of glutaraldehyde on gold electrode has been developed for determination of IgM. Alkaline phosphatase (ALP)-conjugated monoclonal anti-mouse immunoglobulin M (IgM) antibody was selectively bound to IgM molecules onto the surface of the electrode. Electrochemical response arising from the catalytic reaction of alkaline phosphatase enzyme. Its reaction with various phosphates such as p-aminophenyl phosphate and p-nitrophenyl phosphatase (p-NPP) generates the electrochemically active products p-aminophenol (p-AP) and p-nitrophenol (p-NP), respectively.