Thymic output changes in children with clinical findings signaling a probable primary immunodeficiency.
Makaleler > Tam Makale · 2019
Yazarlar: Neslihan KARACA, SE Akarcan, Güzide AKSU, N Kütükçüler, Elif AZARSIZ
TURKISH JOURNAL OF PEDIATRICS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2019, cilt 61, ss 885-885
DOI: 10.24953/turkjped.2019.06.010
Özet
Thymic maturation evaluation is inevitable for patients with clinical and laboratory findings for a primary immunodeficiency, as the T cell-immunodeficiencies are the most severe type. In this study, we aimed to show the usage of T cell surface molecule "CD31" for the evaluation of thymic output in patients (n: 66) with a large spectrum of findings signing a probable primary immunodeficiency. Besides the classical clinical and laboratory approach for these patients, T cell subpopulations as naive, memory, recent thymic emigrant cells were also investigated. The humoral immunodeficiency (34.8%), combined immunodeficiency (34.8%) and cardiopathy (7.6%) were the most frequent diagnosis groups. CD4(+)CD45RA(+) naive T-cells percentages (p: 0.011) and absolute counts (p: 0.004) and absolute CD4(+)CD45RA(+)CD31(+) RTE (recent thymic emigrant) cell counts (p: 0.007) were significantly lower in combined immunodeficiency group. Naive T-cells (p: 0.037) and RTE cells (p: 0.032) were also lower in patients who had cardiac surgery in the past. In conclusion, flow cytometric CD31(+)thymic naive RTE cell evaluation may provide rapid clinical information especially on T-cell immune dysfunction and CD4(+)CD45RA(+)CD31(+) RTE cells may be used as an alternative to TRECs in the diagnosis of combined immunodeficiencies.