Immunomodulatory Effects of MDA-MB-231-derived Exosome Mimetic Nanovesicles on CD4+ T Cell Line
Makaleler > Tam Makale · 2024
Yazarlar: Mustafa Oztatlici, Alper Tunga Ozdemir, Hulya Oztatlici, Serife Kucukhuyuk, Rabia Bilge Ozgul Ozdemir, Hilmi ORHAN, Kemal Ozbilgin
EURASIAN JOURNAL OF MEDICINE AND ONCOLOGY, ESCI İndekslerine Giren Dergi, Uluslararası, Wos, 2024, cilt 8, ss 40-48
Özet
Objectives: The aim of this study is to investigate the immunomodulatory effects of MDA-MB-231 cells or MDA-MB231-derived exosome-mimetic nanovesicles (NVs) on CD4+ Jurkat cells. Methods: NVs were produced by the breakdown of MDA-MB-231 cells and the characterization of generated NVs were performed by using direct-ELISA and Flow cytometry methods. We co -cultured CD4+ Jurkat cells with MDA-MB-231 cells or MDA-MB-231-derived NVs for 48 h. Subsequently, expressions of pro -inflammatory and anti-inflammatory cytokines, and related transcription factors of CD4+ Jurkat cells were evaluated by qPCR method. Results: Clustering, which is the indicator of activation, was not seen in the CD4+ Jurkat cells co -cultured with MDAMB-231 cells. However, CD4+ Jurkat cell clusters were observed in the co -culture experiments with all NV concentrations. In addition, it was determined that the expressions of pro -inflammatory cytokines significantly increased while the expressions of anti-inflammatory cytokines was dramatically decreased in the NV -treated groups. On the contrary, opposite results were obtained in CD4+ Jurkat cells co -cultured with MDA-MB-231 cells. Moreover, TNF-alpha and Gata3 expressions were decreased in all groups. Conclusion: These preliminary findings from in -vitro experiments suggested that NVs could be a potential tool in cancer immunotherapy, but our data need to be supported by more comprehensive studies.