Enzymes on chemical gardens: Chemobrionics-based electrochemical biosensor
Makaleler > Tam Makale · 2024
Yazarlar: Umut Mengulluoglu, Bahar Aslanbay Guler, Simge Er Zeybekler, Esra İMAMOĞLU, Dilek Odaci
Microchemical Journal, SCI İndekslerine Giren Dergi, Uluslararası, Wos, 2024, cilt 205, ss 111192-111192
DOI: 10.1016/j.microc.2024.111192
Özet
The relationship between biological materials and the immobilization matrix is a critical point in attaching biomolecules onto transducers in the preparation of biosensors. Various chemical molecules are used to conjugate biological compounds in the design of biofunctional surfaces. Among them, chemobrionics (CBCs) are good alternatives as an immobilization matrix to improve performance parameters such as linear range, limit of detection (LOD), stability, reproducibility, etc. Here, CBCs are used to develop enzymatic biosensor, and glucose oxidase (GOx) is selected as a model enzyme. Firstly, CBC is functionalized using (3-Aminopropyl)triethoxysilane (APTES) to obtain free amine groups for immobilization of GOx via covalent bonds. The synthesized CBC-APTES is used as a matrix for the conjugation of GOx using glutaraldehyde as a crosslinker. The proposed CBC-APTES/ GOx is formed on the glassy carbon electrode, and its performance is checked for glucose detection. The linear range is 0.025-1.25 mM with an LOD of 0.024 mM glucose. After finding no effect of some potential interference on the current response, CBC-APTES/GOx is successfully applied to analyze glucose in artificial samples such as serum, urine, sweat, and saliva. The objective here is to outline the integration of CBC in biosensor preparation, which has been successfully carried out for the first time in the literature.