Catechol-Attached Polypeptide with Functional Groups as Electrochemical Sensing Platform for Synthetic Cannabinoids
Makaleler > Tam Makale · 2019
Yazarlar: Ceren Durmus, Eda Aydindogan, Zinar Pınar GÜMÜŞ, Takeshi Endo, Shuhei Yamada, Hakan COŞKUNOL, Suna TİMUR, Yusuf Yağcı
ACS APPLIED POLYMER MATERIALS, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2019, cilt 2, ss 172-177
Özet
Herein, we first constructed a functional surface using a catechol-attached polypeptide (CtP) for the detection of JWH-018 (N-4-hydroxypentyl metabolite). K2 antibody was then incorporated to the polymer via covalent cross-linker. Step-by-step modifications on the glassy carbon electrode surface were characterized by electrochemical measurements such as differential pulse voltammetry, cyclic voltammetry, impedance spectroscopy, and X-ray photoelectron spectroscopy. Linearity and the limit of detection for JWH-018 (N-4-hydroxypentyl metabolite) were determined as 10-500 ng/mL with an equation of y = 0.0018x + 0.136 (R-2 = 0.993) and 5.892 ng/mL, respectively. The selectivity of the biosensor was evaluated with different interfering molecules (methamphetamine, codeine, and cocaine). Finally, the biosensor was successfully used in the determination of JWH-018 (N-4-hydroxypentyl metabolite) in spiked synthetic urine samples, and a high-performance liquid chromatography (HPLC) system was used as a reference method to confirm the sample application. The results show that this biosensor platform can be applied to detect other JWH series of synthetic cannabinoids with high sensitivity and accuracy.