Determination of genetic diversity between natural and cultured populations of Common Dentex (Dentex dentex) fish in the East Aegean Sea

Articles > Article · 2023

Authors: ALİ KAYACI, Mehmet Fatih Can, YUSUF GÜNER, Fatih GÜLEÇ

OCEANOLOGICAL AND HYDROBIOLOGICAL STUDIES, Journal Indexed in SCI Expanded, International, WoS, 2023, vol 52, pp 52-67

DOI: 10.26881/oahs-2023.1.04

Summary

<p>Common&nbsp;&nbsp;dentex&nbsp;&nbsp;(Dentex&nbsp;&nbsp;dentex)&nbsp;&nbsp;is&nbsp;&nbsp;a&nbsp;&nbsp;commercial&nbsp;&nbsp;species&nbsp;of&nbsp;fish&nbsp;that&nbsp;is&nbsp;a&nbsp;highly&nbsp;valuable&nbsp;food&nbsp;source&nbsp;living&nbsp;naturally near Mediterranean and Atlantic Coasts. Therefore, monitoring&nbsp;and&nbsp;maintaining&nbsp;common&nbsp;dentex&nbsp;habitats&nbsp;are&nbsp;of high importance. A total of 53 specimens were collected from&nbsp;6&nbsp;natural&nbsp;habitats&nbsp;(from&nbsp;the&nbsp;Eastern&nbsp;Aegean&nbsp;and&nbsp;from&nbsp;the Antalya coast, which is the closest coast to the Western Mediterranean),&nbsp;and&nbsp;one&nbsp;aquaculture&nbsp;facility&nbsp;in&nbsp;the&nbsp;Eastern&nbsp;Aegean Sea. The mitochondrial Cytochrome Oxidase I (COI) gene&nbsp;was&nbsp;used&nbsp;to&nbsp;detect&nbsp;a&nbsp;total&nbsp;of&nbsp;26&nbsp;haplotypes.&nbsp;Along&nbsp;with&nbsp;&nbsp;the&nbsp;&nbsp;COI&nbsp;&nbsp;gene&nbsp;&nbsp;sequence,&nbsp;&nbsp;real-time&nbsp;&nbsp;PCR&nbsp;&nbsp;and&nbsp;&nbsp;high-resolution&nbsp;melting&nbsp;analyses&nbsp;were&nbsp;performed&nbsp;as&nbsp;rapid&nbsp;and&nbsp;inexpensive&nbsp;alternatives.&nbsp;Sequence&nbsp;analysis&nbsp;showed&nbsp;that&nbsp;the&nbsp;&nbsp;highest&nbsp;&nbsp;haplotype&nbsp;&nbsp;diversity&nbsp;&nbsp;was&nbsp;&nbsp;obtained&nbsp;&nbsp;from&nbsp;&nbsp;the&nbsp;&nbsp;aquaculture&nbsp;facility&nbsp;in&nbsp;Karaburun&nbsp;and&nbsp;Antalya&nbsp;Locations,&nbsp;Turkey.&nbsp;&nbsp;Analysis&nbsp;&nbsp;of&nbsp;&nbsp;Molecular&nbsp;&nbsp;Variance&nbsp;&nbsp;(AMOVA)based on&nbsp;the&nbsp;haplotype&nbsp;frequencies&nbsp;resulted&nbsp;in&nbsp;92.54%&nbsp;genetic&nbsp;variation&nbsp;&nbsp;within&nbsp;&nbsp;localities&nbsp;&nbsp;and&nbsp;&nbsp;7.46%&nbsp;&nbsp;genetic&nbsp;&nbsp;variation&nbsp;&nbsp;between/among&nbsp;localities.&nbsp;The&nbsp;mean&nbsp;fixation&nbsp;index&nbsp;(Fst) was calculated as 0.0746 (p &lt; 0.001). Genetic distances were primarily&nbsp;in&nbsp;collaboration&nbsp;with&nbsp;geographical&nbsp;distances&nbsp;and&nbsp;were efficiently confirmed by high resolution melting (HRM) analysis.&nbsp;The&nbsp;results&nbsp;will&nbsp;be&nbsp;valuable&nbsp;in&nbsp;monitoring&nbsp;and&nbsp;maintaining natural habitats as well as aquaculture facilities where common dentex are grown.ISSN 1730-413XeISSN 1897-3191Oceanological and Hydrobiological Studies</p><p><br></p>

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