Development of an Amperometric Glucose Biosensor Based on the Immobilization of Glucose Oxidase on the Se-MCM-41 Mesoporous Composite
Makaleler > Tam Makale · 2018
Yazarlar: Sabriye YUŞAN, Mokhlesur M. RAHMAN, Nasir MOHAMAD, Tengku M. ARRIF, Ahmad Zubaidi A. LATIF, Mohd M. A. AZNAN, Wan Sani B. Wan NIK
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2018, cilt 2018, ss 1-8
DOI: 10.1155/2018/2687341
Özet
A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 x 10(-5) to 2 x 10(-3) M. Under optimal conditions, the biosensor exhibits high sensitivity (0.34 mu A.mM(-1)), low detection limit (1 x 10(-)(4)M), high affinity to glucose (K-m = 0.02 mM), and also good reproducibility (R.S.D. 2.8%, n = 10) and a stability of about ten days when stored dry at +4 degrees C. Besides, the effects of pH value, scan rate, mediator effects on the glucose current, and electroactive interference of the biosensor were also discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.