Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
Makaleler > Tam Makale · 2007
Yazarlar: Raziye Ozturk Urek, Nurdan Kasikara Pazarlioglu
Brazilian Archives of Biology and Technology, Uluslararası, Wos, 2007, cilt 50, ss 913-920
DOI: 10.1590/s1516-89132007000700001
Özet
Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.