Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications
Makaleler > Tam Makale · 2011
Yazarlar: Fatma Bilge Emre, Fulya Ekiz, Abidin Balan, Sinan Emre, Suna TİMUR, Levent Toppare
SENSORS AND ACTUATORS B-CHEMICAL, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2011, cilt 158, ss 117-123
DOI: 10.1016/j.snb.2011.05.052
Özet
Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at -0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (K(m) and I(max)) and surface morphologies. The biosensor was tested on real human blood serum samples. (C) 2011 Elsevier B.V. All rights reserved.