Electrochemical detection of enzyme labeled DNA based on disposable pencil graphite electrode
Makaleler > Tam Makale · 2005
Yazarlar: Pınar KARA, Kadriye Arzum ERDEM GÜRSAN, S GIROUSI, M OZSOZ
Journal of Pharmaceutical and Biomedical Analysis, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2005, cilt 38, ss 191-195
DOI: 10.1016/j.jpba.2004.12.011
Özet
Electrochemical biosensor for the detection of DNA hybridization using the reduction signal of a-naphthol is described. A pencil graphite electrode was used as a working electrode. Capture probes were covalently attached on to the pencil graphite electrode surface (PGE) at the 5' end amino group by using N-(dimethylamino)propyl-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (NHS) as a coupling agent on to PGE. After capture probe immobilization on to PGE surface; probe was hybridized with complementary biotinylated oligonucleotide. Alkaline phosphatase labeled with extravidin (Ex-AP) binds to biotinylated hybrid via biotin-avidin interaction. alpha-Naphthyl phosphate (alpha-NAP) was added and the reaction between alkaline phosphatase (AP) and alpha-NAP was occurred consequently as a substrate of AP, alpha-NAP reduction signal was obtained from this reaction, at -0.100 V by using differential pulse voltammetry (DPV). Other experimental parameters were studied such as; optimizations of hybridization time, and the concentrations of capture probe, biotinylated oligonucleotide and enzyme. (c) 2005 Elsevier B.V All rights reserved.