Cysteamine–palladium complex ([Pd(μ-OAc)(ppy)]2, ppy:2-phenylpyridine, PhMe)-modified peroxidase biosensor immobilized on a gold electrode
Makaleler > Tam Makale · 2013
Yazarlar: Erol AKYILMAZ, M. Emin Gunay, Engin Asav, Rukiye Gumusada
Artificial Cells, Nanomedicine, and Biotechnology, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Scopus, 2013, cilt 42, ss 413-417
DOI: 10.3109/21691401.2013.815193
Özet
A new peroxidase biosensor was developed using cysteamine-palladium complex-modified gold electrode. The principle of the measurements is based on monitoring increase in the oxidation potential of palladium complex (at + 0.47 V vs Ag/AgCl) using amperometric detection. In the optimization studies of the biosensor, effects of enzyme amount, palladium complex amount, and duration of SAM formation on biosensor responses were investigated to optimize the bioactive layer. The biosensor has a fast response time of less than 10 s to hydrogen peroxide (H2O2), with a linear range of 5.0 × 10(- 6) to 150 × 10(- 6) M and a detection limit of 3.38 × 10(- 6) M.