The relationship of the peroxisome proliferator-activated receptor-gamma 2 exon 2 and exon 6 gene polymorphism in Turkish type 2 diabetic patients with and without nephropathy
Makaleler > Tam Makale · 2007
Yazarlar: Mehmet ERDOĞAN, MUAMMER KARADENİZ, Fatma Zuhal EROĞLU, Burçin KAYMAZ, Nur SELVİ GÜNEL, CENGİZ YILMAZ
Diabetes Research and Clinical Practice, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2007, cilt 78, ss 355-359
DOI: 10.1016/j.diabres.2007.06.005
Özet
Background: Peroxisome proliferator-activated receptor gamma (PPARgamma) has recently been shown to be associated with type 2 diabetes. We aim to investigate Turkish type 2 diabetic patients with/without diabetic nephropathy and healthy group and examined the contribution of the G/C exon 2 and T/C exon 6 of the PPARgamma gene polymorphism to the development of diabetic nephropathy. Methods: The PPARgamma genotypes were determined retrospectively in 43 patients with nephropathy and 48 without nephropathy and a control group of 50 healthy individuals. Genotyping of the G/C exon 2 and T/C exon 6 of the PPAR-gamma gene polymorphism for all individuals was performed by melting curve analysis of the generated amplicons after real-time online PCR. Results: This genotype (exon 2 and exon 6) distribution did not differ between control subjects and type 2 diabetic patients. The genotype frequencies and allele exon 2 were CC, 100%; GC, 0%; GG, 0% and C, 100%; G, 0% in diabetic patients with nephropathy versus CC, 97.9%; GC, 2.1 %; GG, 0% and C 98.9%, G 1.1 % in those without nephropathy. Genotype exon 6 frequencies in diabetic patients with nephropathy were (T/T) 0%, (T/C) 14%; (C/C) 86% versus (G/G) 0%; (G/C) 2.1 %; (C/C) 97.9% in those without nephropathy. The PPARgamma exon 2 and exon 6 genotype and allele frequencies were not different between diabetic patients with and without nephropathy. Conclusions: PPARgamma exon 2 and exon 6 gene polymorphism is not associated with the development of diabetic nephropathy in Turkish type 2 diabetic patients. (c) 2007 Elsevier Ireland Ltd. All rights reserved.