An Electrochemical DNA Biosensor for the Detection of DNA Damage Caused by Radioactive Iodine and Technetium
Articles > Article · 2007
Authors: Pınar KARA, Kağan Dağdeviren, Mehmet Özsöz
\n Volume: 31, Issue: 3\n 243-249\n \n, International, 2007, vol 31, pp 243-249
Summary
A DNA damage process by radioactive iodine (I-131) and technetium (Tc-99m) is described. The procedure relies on a label-free electrochemical DNA biosensor. Fish sperm double stranded DNA was immobilized onto a carbon paste electrode (CPE) by electro deposition and the changes to the guanine oxidation signal were observed upon exposure to radioactive I-131 and Tc-99m by differential pulse voltammetry (DPV). The changes to the signal of guanine after exposure to therapeutic doses of Tc-99m and I-131 showed conformational changes in DNA molecules.