Impedimetric Detection of Free Fatty Acids in Patient Serum Using Commercially Available Screen-Printed Carbon Electrode
Makaleler > Tam Makale · 2026
Yazarlar: İsmail ORAN, Halil İbrahim ÖZDEMİR, Turgay Yılmaz Kılıç, Hilmiye Deniz Ertuğrul Uygun, Hakan Gökalp Uzun, Barış Kılıçaslan, Evrim ŞİMŞEK, Yusuf Ali ALTUNCI, Şadiye MIDIK, Ali Murat Ergin
Chemosensors, Uluslararası, Wos, 2026, cilt 14, ss 53-53
DOI: 10.3390/chemosensors14030053
Özet
Objective: The performance of chrono-impedance measurement, a novel electrochemical method for determining free fatty acids (FA), was evaluated in a real-world clinical setting. Methods: Patients presenting to the emergency department with chest pain or discomfort were included. Routine diagnostic tests were performed in accredited laboratories. Chrono-impedance was measured using a screen-printed carbon electrode connected to a dedicated potentiostat. Serum total free-FA levels were determined by gas chromatography with flame ionization detection. Results: Among 104 patients, 21 received a specific diagnosis, while the remaining 83 patients were discharged with non-specific pain. Mean free-FA level was 0.9 ± 0.6 mM. Palmitic, linoleic, stearic, oleic, and arachidonic acids accounted for 74.9% of total free FAs. Impedance plots showed a characteristic logarithmic increase over time for all patients. When instantaneous impedance values at four different time points (10, 100, 376.6, and 500 s) were examined, a significantly strong correlation was observed between impedance and FA molarity (r = 0.8312, 0.9897, 0.9947, and 0.9951) and FA weight (r = 0.9572, 0.9878, 0.9996, and 0.9998), respectively. Conclusions: Chrono-impedance demonstrated a very high correlation with total free-FA levels in real patient samples.