Toxicological and inflammatory effects of engineered nanoparticles and diesel exhaust particles on human mesothelial cells
Bildiriler > Özet Bildiri
Yazarlar: Canan Demir, Demet Tasdemir, Sedat Ilhan, Ahmet Feridun Isik, Hasan Bayram
Airway Cell Biology and Immunopathology, , , Uluslararası, Özet Bildiri, ss PA3918-PA3918
DOI: 10.1183/1393003.congress-2017.pa3918
Özet
Aim:Since data on environmental and engineered nanoparticles9(NP) effects on pleural cells is scarce, we aimed to evaluate the effects of multi-walled carbon nanotubes (MWCNT) and diesel exhaust particles(DEP) on both benign mesothelial cell line(MeT-5A) and mesothelioma cell line(CRL-5820), first time in the literature.Method:MeT-5A and CRL-5820 cell lines were incubated for 24 hours with crocidolite, MWCNT and DEP suspensions at doses of 0,50,100 and 300 μg/ml.Cell viability MTT assay, lactate dehydrogenase(LDH), interleukin(IL)-8 and granulocyte macrophage-colony stimulating factor(GM-CSF) levels were studied.Results:MWCNT(300 µg/ml) suppressed cell viability only in MeT5A cells(p<0.01), besides DEP(100, 300µg/ml) suppressed cell viability both in MeT5A and in CRL-5820 cells(p<0.05, p<0.001).Crocidolite induced cell viability in CRL-5820 cells at doses of 100(p<0.05) and 300µg/ml(p<0.001) and in MeT5A cells at doses of 50(p<0.05),100(p<0.001) and 300(p<0.001) µg/ml significantly.DEP(100 and 300 μg/ml) increased LDH levels only in CRL-5820 cells, while MWCNT did not affect LDH release. Crosidolite induced LDH release in both cell types. While MWCNT only reduced IL-8 release from CRL-5820 cells, it reduced GM-CSF levels in both cell groups.DEP increased release of IL-8 and GM-CSF from MeT5A cells, but suppressed these cytokine levels in CRL-5820 cells. Crosidolite increased IL-8 release in both cell types but increased GM-CSF levels only in CRL-5820 cells. Conclusion: Our findings suggest that MWCNT and DEP may have toxic effects on both benign and malignant human mesothelial cells, may affect the inflammatory response, and may pose a risk for pleural diseases.