Identification of gene expression profiles in Leishmania major infection by integrated bioinformatics analyses.
Makaleler > Tam Makale · 2020
Yazarlar: Ö Ulusan, U Mert, A Sadıqova, S Öztürk, Ayşe CANER
ACTA TROPICA, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2020, cilt 208, ss 105517-105517
DOI: 10.1016/j.actatropica.2020.105517
Özet
Gene expression profiling in mouse models of leishmaniasis has given useful information to understand the molecular pathways active in lesions and to discover new diagnostic/therapeutic targets. Although the host response plays a critical role in protection from leishmaniasis and promoting disease severity, there are still unexplained aspects in the mechanism of non-healing cutaneous lesions, which need biomarkers for both targeted- therapy and diagnosis. To address this, transcriptional profiling of the skin lesions obtained from BALB/c mice infected with Leishmania major and healthy skin from naive mice were evaluated by bioinformatics analysis, and then the results were validated by Revers Transcriptase-PCR. Five genes among the up-regulated differentially expressed genes named FCGR4, CCL4, CXCL9, Arg1 and IL-1 beta were found to have relatively high diagnostic value for CL due to L. major. Pathway analysis revealed that Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) signaling pathways are active in cutaneous lesions, providing new insights for the understanding and treatment of leishmaniasis.