Evaluation of Telomerase Activity in Gingival Fibroblasts of Cyclosporine-Treated Patients
Makaleler > Tam Makale · 2008
Yazarlar: Çığır BİRAY AVCI, Nejat NİZAM, Cumhur GÜNDÜZ, Fatma Şule SÖNMEZ, C. Cavdar, F. Saglam, K. Atılla, T. Camsarı
Transplantation Proceedings, SCI Expanded İndekslerine Giren Dergi, Uluslararası, Wos, 2008, cilt 40, ss 184-185
DOI: 10.1016/j.transproceed.2007.11.065
Özet
Gingival overgrowth (GO) is a common side effect following administration of cyclosporin A (CsA). Various case reports have shown that squamous cell carcinomas could arise in GO induced by CsA and phenytoin. It is also known that human telomerase activated in about 90% of cancers is mainly composed of hTR, hTERT, and TPI. The aim of this study was to investigate the potential role of telomerase activity in the pathogenesis of CsA-induced GO. Included in the study were 9 patients on CsA: 4 with and 5 without GO. Gingival tissues were obtained during gingivectomy or flap procedures; gingival fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10,000 U/mL penicillin, 10 mg/mL streptomycin, 2 mmol/L L-glutamine, and 10% heat-inactivated fetal bovine serum at 37 degrees C under a humidified 95% air virgule 5% CO2 atmosphere. Quantitative detection of hTERT mRNA was performed with the commercially available LightCycler Telo TAGGG hTERT Quantification Kit using real-time online PCR. The hTERT mRNA expression was positive in one patient, while hTERT mRNA expression was negative in the others. Because results indicated that there may be a relationship between CsA-induced GO and positive telomerase activity, detailed studies should be performed to confirm the present findings.