Effect of Forced Nicotine on Oxytocin and Vasopressine Levels in Striatum and Hippocampus in Nicotine Preferring Rat Lines
Bildiriler > Tam Metin Bildiri · 2022
Yazarlar: Zümra Kul, Servet Kızıldağ, Fulya TUZCU ÜNAL, Lütfiye KANIT, Burcu BALKAN, Ayşegül KESER
Turkish Society of Physiological Sciences, 47th Turkish Physiology Congress, Türkiye, Antalya, Uluslararası, Poster, Tam Metin Bildiri, 2022-2022, Biological Abstracts, ss 24-24
DOI: 10.1111/apha.13910
Özet
<p>Effect of Forced Nicotine on Oxytocin and Vasopressine Levels in Striatum and Hippocampus in Nicotine Preferring Rat Lines</p><p><br></p><p>AIM: This study aimed to determine the differences in hippocampal and striatal oxytocin and arginine-vasopressin (AVP) levels in Nicotine-preferring (NP) (male/female) and control (male/female) animals during chronic oral nicotine exposure.</p><p>METHODS: NP line is developed by selective breeding at Ege University Center for Research on Laboratory Animals. This study is a continuation project of previous project (TDK-2020-21454). Same animals were used in both projects; there were 4 groups (n=10/group): Control Male (CM), Control Female (CF), NP Male (NPM), NP Female (NPF). All groups received forced nicotine administration in drinking water for 4 weeks. In the previous project, we analyzed differences in empathy-like behavior of NP and control groups during baseline and chronic nicotine exposure. In this project, animals were decapitated following behavioral testing; striatum and hippocampus were dissected and stored at -80°C. Oxytocin and AVP levels in samples were measured by ELISA. One-way ANOVA and post-hoc Tukey tests were performed using SPSS for statistical analysis.</p><p>RESULTS: There were significant differences between the groups (p<0.001). Hippocampal oxytocin levels were higher in NPM compared to CM (p=0.002), in NPF compared to CF (p=0.05), in NPM compared to NPF (p=0.05), and in NPM compared to CF (p<0.001). Striatal oxytocin levels in NP were lower than controls (p=0.05). Striatal oxytocin levels in NPF were lower than CM (p=0.02). Hippocampal AVP levels in NP were higher than controls (p=0.005). Hippocampal AVP levels in NPM were higher than CF (p=0.02). Striatal AVP levels were not different between groups.</p><p>CONCLUSION: Alterations in brain oxytocin and AVP levels of NP animals may underlie the changes observed in empathy-like and social behavior of these animals. These differences may contribute to the susceptibility of NP rats to nicotine addiction.</p><p>Supported by Ege University, Scientific Research Projects Commission (TYL-2020-22397).</p>