Superparamagnetic iron oxide nanoparticle (SPION) mediated in vitro radiosensitization at megavoltage radiation energies
Articles > Article · 2018
Authors: Esra Korkmaz Kıraklı, Gokhan Takan, Sinan HOCA, Fazilet Zümrüt BİBER MÜFTÜLER, Ayfer YURT KILÇAR, Emine Serra KAMER
Journal of Radioanalytical and Nuclear Chemistry, Journal Indexed in SCI Expanded, International, WoS, 2018, vol 315, pp 595-602
DOI: 10.1007/s10967-018-5704-9
Summary
The purpose of this study was to assess SPION's in vitro radiosensitizer effect at 6MV-energies and to calculate nanoparticle enhancement ratio (NER) of SPIONs. Citrate coated-SPION's were synthesized. Trypan-blue, metabolic activity tests were performed. Cell cultures were irradiated at 0,2,4,6,8 Gy at 6MV-energy. Clonogenic survival assays, NER calculations were carried out. SPIONs were biocompatible. NERs were cell-line specific and dose dependent. The highest radiosensitization were seen in radiosensitive MCF-7 and MDAH-2447 cells at 2 Gy (NER:1.49 and 1.39 respectively), in relatively radioresistant MDA-MB-231 cells at 4 Gy (NER:1.20). By increasing doses radiosensitizer effect disappeared. There is possibility that by synergistic effect, SPIONs may cause dose-dependent and cell-line specific radiosensitization at 6MV.