Voltammetric and Impidimetric Detection of Anticancer Drug Mitomycin C and DNA Interaction by Using Carbon Nanotubes Modified Electrodes
Makaleler > Tam Makale · 2015
Yazarlar: Ece Eksin, Hakan KARADENİZ, Kadriye Arzum ERDEM GÜRSAN
Current Bionanotechnology, Uluslararası, 2015, cilt 1, ss 32-36
DOI: 10.2174/2213529401666150218194827
Özet
The interaction of Mitomycin C with dsDNA was performed with differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) by using carbon nanotubes modified single-use pencil graphite electrode (CNTs-PGE) and bare PGE. The passive adsorption process was used to modify the electrode surface by CNTs and then dsDNA was immobilized onto the modified PGEs by a strong chemical interaction between the amine groups of dsDNA and the carboxyl groups of CNTs. The performance of CNT-PGEs was studied by examining the optimum analytical conditions; MC concentration, interaction time, etc. with voltammetric methods and then impedance method was performed to characterize the modification of CNTs onto the electrode surface. Keywords: Biosensor, carbon nanotubes, differantial pulse voltammetry, drug-DNA interaction, electrochemical impedance spectroscopy, mitomycin C.