PREPARATION OF GLUTATHIONE IMPRINTED POLYMER
Makaleler > Tam Makale · 2010
Yazarlar: Ayse Dincer, Figen ZİHNİOĞLU
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, SCI Expanded İndekslerine Giren Dergi, Uluslararası, 2010, cilt 40, ss 188-197
DOI: 10.1080/10826068.2010.488515
Özet
Glutathione imprinted polymer was prepared using 1-vinyl imidazole and ethylene glycol dimethacrylate as the functional monomer and crosslinker, respectively, in dimethyl sulfoxide. The adsorption selectivity of glutathione-imprinted polymer was tested by reduced glutathione, oxidized glutathione, and L-Gly-Leu-Tyr in 30% phosphate buffer (0.01 M, pH 5.0)-70% acetonitrile and binding affinity values were compared. Reusability of molecularly imprinted polymer particles was also investigated. Molecularly imprinted polymer particles were found to be stable and to maintain glutathione adsorption capacity at 95% when washed with methanol-acetic acid (10%) after seven usages. Functional monomer 1-vinyl imidazole and cross linker ethylene glycol dimethacrylate-based glutathione imprinted polymer could be used as solid phase extraction material for recognition of glutathione in biological samples.